Sequence Similarity Searching
CME Credits: 2
Target Audience:
Faculty, researchers, staff and students who want to learn more about searching the NCBI’s suite of databases related to genetics, gene sequences, and gene mapping.
Prerequisite:
None
Description:
This class will focus on determining which BLAST program to use, which database is best to search, and how to set searching parameters. It will also address the use of BLINK for finding the conserved domains in a query sequence and how to display sequences with known 3D structures.
Goals:
To provide a short course on how to search NCBI’s biological and biomedical sciences database. The main aim is to provide efficient methodologies for searching the databases and arriving at specified results.
Objectives:
To present the various search tools available via NCBI in such a manner that they may be used most efficiently to retrieve specific search and data.
Intended Results:
- Determine which BLAST program to use.
- Determine which BLAST database to search.
- Set the searching parameters for the advanced search.
- Understand the results output.
- Find conserved domains in your query sequence that are common to many sequences.
- Display similar sequences with known 3D structures.
- Display a taxonomic tree of all organisms with similar sequences.
